> For the complete documentation index, see [llms.txt](https://swatplus.gitbook.io/io-docs/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://swatplus.gitbook.io/io-docs/introduction-1/hydrology/hydrology.hyd/bio_mix.md). # bio\_mix Biological mixing is the redistribution of soil constituents as a result of the activity of biota in the soil (e.g. earthworms, etc.). Studies have shown that biological mixing can be significant in systems where the soil is only infrequently disturbed. In general, as a management system shifts from conventional tillage to conservation tillage to no-till there will be an increase in biological mixing. SWAT+ allows biological mixing to occur to a depth of 300 mm (or the bottom of the soil profile if it is shallower than 300 mm). The efficiency of biological mixing is defined by the user and is conceptually the same as the mixing efficiency of a tillage implement. The redistribution of nutrients by biological mixing is calculated using the same methodology as that used for a tillage operation. Biological mixing is performed at the end of every calendar year. --- # Agent Instructions This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com. ## Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter, and the optional `goal` query parameter: ``` GET https://swatplus.gitbook.io/io-docs/introduction-1/hydrology/hydrology.hyd/bio_mix.md?ask=&goal= ``` `ask` is the immediate question: it should be specific, self-contained, and written in natural language. `goal` is optional and describes the broader end goal you are ultimately trying to accomplish on behalf of the user. GitBook uses it to tailor the answer towards what is most useful for that goal. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.